ARC Home I SJAR Home I About SJAR I Article Order Info. I Contribution to SJAR

Sudan Journal of Agricultural Research

 Elamein, H.M.M.,  Ali, A.M., Garg, M., Tanaka, H.., Tsujimoto, H.

Abstract

Karyotypes of P. schweinfurthii and P. glaucum using acetocarmine condensation pattern, centromeric satellite DNA and 45S        rDNA sequences were analyzed. The chromosome complement of P. schweinfurthii consisted of four metacentric (I, III, IV and V) and three sub-metacentric (II, VI and VII) chromosomes. On the other hand, the chromosome complement of P. glaucum consisted of four sub-metacentric (I, III, IV, V and VI), one metacentric (II) and one sub-telocentric (VII) chromosomes. The chromosome length ranged between 2.20 and 4.90 µm in P. schweinfurthii compared to 2.95 and 4.70 µm in P. glaucum. Large heterochromatin regions, mainly located at centromeric and telomeric regions in most chromosomes of the two species were observed after staining with acetocarmine. Marker bands were clearer in P. schweinfurthii than in the P. glaucum. Sites of 45S rDNA sequence were found at two metacentric chromosome pairs in P. schweinfurthii, one sub-metacentric and one sub-telocentric chromosome pair in P. glaucum. The intensity of the 45S rDNA signal was  higher in P. schweinfurthii than in P. glaucum. The results indicated that combining both centromeric satellite and 45S rDNA sequences was useful in differentiating some chromosomes of the two species.

Sudan Journal of Agricultural Research Vol. 13  (2009) PP. 1-12