Agricultural Research Corporation (ARC) - Sudan
Proceedings of the Meetings of the National Crop Husbandry Committee - NCHC
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Propagation of grapefruit by shoot tip micrografting
Hamaraie M. A. Ali , Osman M. Elamin1 and Mohamed A. Ali
Abstract:
This study was conducted to develop an in vitro technique for
micrografting of grapefruit on sour orange rootstock during August, 2001
to May, 2002. The best in vitro germination of rootstock seed was
obtained when both seed coats (hard and soft) were removed before in
vitro culturing on a nutrient medium. The success of micrografting was
affected by the age of rootstock seedling, light incubation conditions,
source and method of placement of meristem tips and concentration of
sucrose in the nutrient medium. High frequency of successful micrografts
was obtained when the rootstock seedlings were germinated under darkness
till the age of two weeks and then incubated in the same condition for
two to three weeks after grafting. Plants grown in the greenhouse were
the best source for meristem tips. Placement of scion on an inverted T
incision at the point of decapitation was found to be the most suitable
method of micrografting. The frequency of successful micrografts
improved significantly when Murashige and Skoog medium was supplemented
with 7.5% sucrose compared with normal sucrose concentration (3%).
Proceedings of the Meetings of the National Crop Husbandry Committee 37th (2005) pp. 220-227
Proceedings of the Meetings of the National Crop Husbandry Committee - Sudan
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