Agricultural Research Corporation (ARC) - Sudan
Proceedings of the Meetings of the National Crop Husbandry Committee - NCHC
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Optimal dose rate of gamma irradiation and EMS concentration for mutation induction on shoot tip
of Banana cv. Grand Nain
Mohamed Ahmed Ali
Abstract:
This work was initiated in the year 1997 to develop an in vitro
technique suitable for mutation induction on banana cultivar Grand Nain
using physical and chemical mutagens. The first experiment was
established to determine the optimal dose range of gamma source
radiation for mutagenic induction on the banana cv. Grand Nain for
subsequent mutation breeding in the crop. LD50 was established by
irradiation of shoot tip explants by 33.6 Gy based on the survival of
explants and shoot proliferation. The second experiment was established
to test the best method of irradiation comparing acute and split methods
with the aim of increasing the chance of variability with a reduced
lethal effect on explants. The acute method of irradiation was better
than the split one based on the survival of explants and shoot
proliferation. Chemical mutagenesis can be induced using .2%
ethylmethanesulphonate (EMS) with or without DMSO on in vitro grown
shoot tip explants of cv. Grand Nain.
Proceedings of the Meetings of the National Crop Husbandry Committee 37th (2005) pp. 228-233
Proceedings of the Meetings of the National Crop Husbandry Committee - Sudan
Copyright©2008 National Crop Husbandry Committee
